the use of a reader calibrated with primary NIST and NMI standards. Instrument settings are listed in Table 1. $913. x. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. #S1205. …200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. 00. easy to use and read multiple plates very quickly. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Both readers were heated to 37ºC prior to setting up the assay. #i3X) with: SoftMax Pro 7. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 96-, 384-, and 1536-well microplates. Packed 25 plates per sleeve, 2 sleeves per case. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. Related products. Fluorescent reading data were analyzed by non-linear fitting (GraphPad Prism, GraphPad Software Inc. Well scans from the Gemini EM Reader illustrating serial dilution of CHO cells using the Live Cell assay from Molecular Probes Live/Dead (Calcein AM/Ethidium Homodimer-1) assay kit. . Before you do a bottom read, or use high profile (6-well, 12-well, 24-well, or 48-well) plates, you must remove the plate adapter from the plate drawer. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate. 2. 130009WThe SpectraMax Paradigm reader with HTRF Detection Cartridge offers high-throughput screening capability with excellent read times and Z’ factors. Cell viability는 세포막 Integrity 또는. Outer wells aligned for proper viewing with FLIPRIdeal for miniaturized 384 well fluorescent homogeneous assaysNot treated (or medium binding)… Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. readers are the industry’s most cited instruments and have empowered life science researchers to advance protein and cell biology, breaking the barriers to novel, landmark discoveries. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate. $245. " SpectraMax M5 micro plate reader is a multi mode plate reader (FLU, LUMN . The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. 384-Well Fully Skirted Plates, Barcoded, including Armadillo 384-Well PCR plates. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. When the user applies a set temperature of 37°C, the top is heated to 37. SpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903) Purified luciferase, Promega (Cat# E1701). plated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. x. There is no product in the shopping cart, buy it!. 5in (46. What is the minimum well volume needed to run a 384 well plate? Minimum volume depends on the solution (curved vs. DynaPro ™ Plate Reader III. 1 fmol luciferase per well. 0 . UV and visible absorbanceLocate the User Guide on your SoftMax Pro computer for version 6 and version 7. The. Page 1 of 8 . Ideal for miniaturized 384 well fluorescent. x. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. A ten-fold serial dilution of purified luciferase in PBS with 0. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. Fluorescence Microplate Reader Comparison Chart. Molecular Devices deliver high throughput while showing good correlation with manual patch clamping data. Molecular Devices has developed the FLIPR Membrane Potential Assay Kits to maximize. 96/384-well plate formats. Industry standard disposable (choose from recommended list). ©2010 Molecular Devices, Inc. Two high-energy xenon flash lamps: Reading capabilities. 96- and 384-well microplates. x. This reader provides visible absorbance, top-reading fluorescence intensity, and glow luminescence modes in 96- to 384-well plates with wavelength selection options from 340-650 nm. 1. Find regional support. 2. Specialized microplate readers are available that enable HTRF (Homogeneous Time-Resolved Fluorescence) experiments and analyses. Introduction. No accessories are needed and SoftMax® Pro Software. Summary of Contents for Molecular Devices SpectraMax M2. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. X. Supplier: Molecular Devices. 6 to 384-well plates: 96-well plates: 1. explore more. To prevent evaporation, the microplate was covered with a qPCR seal. Both 96- and 384-well assay formats yielded similar linearity and dynamic range, indicating the suitability of the dual-luciferase assay and SpectraMax i3x system for both formats. They are also fully compatible with the FlexStation 3 Multi-Mode Microplate Reader. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent. The fluorescent intensity of each well was measured by using a fluorescence plate reader (SpectraMax Paradigm, Molecular Devices, Sunnyvale, CA, USA) at 588-nm excitation and 633-nm emission. Gemini XPS Reader scans. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Formats available are strip washers, full plate washers and combination washer-dispensers. Instruments with a 96- or 384-well format will accommodate the most commonly used plates. In addition, SoftMax Pro SoftwareBelow is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. x. With optimized reagents, option to operate the. Molecular Devices SpectraMax Plus 384 Absorbance Reader Details. Ultra-cooled Photomultiplier Tube and : Silicon photodiode. For more sample throughput, the system canThe SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 3% in white 96- and 384-well microplates Time. 190. x. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. 1. Kinetic assays were read every 20-28 seconds for one hour,measurements in compatible Molecular Devices® microplate readers. It is also important to note that organoid growth patterns are relatively consistent from 24- to 384-well plates. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. 384-well microplate compatible Well read area range: H7 to K22 Figure 1-3 24-Well Micro-Volume Sample Slide Figure 1-4. x. research needs. Recertification service. Select the Driver tab. 96 deep well plate: packed in sleeves of 6 , 24 per case. The StakMax® Microplate Handling System is a powerful, compact, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers for improved process flow and higher throughput. 0200-6262. Description. 2. firefly luciferase in 96- and 384-well top read Dynamic range: > 6 decades Cross-talk: < 0. 303 unable to cal dark current. 0 OD for our latest microplate readers, SpectraMax® iD3, iD5, ABS, and ABS Plus readers, and up to 2. Microplate is validated by molecular devices for the FLIPR reader. FLIPR Calcium 4 Assay Kit (Molecular Devices, San Jose, CA, USA). The narrow bandwidths used by the model increase measurement accuracy and linearity over a wide. 5 pM 2. Learn more about SpectraMax Plus 384 Microplate Reader >>Rating: 5. 340 PC384. ". Plus 384. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. #PLUS 384) UV-transparent 96-well microplates (Corning cat. 7. 2. the Molecular Devices SPECTRAmax® 190 and 250 plate. Analyst, M5, Flex 3, and SpectraMax L readers work differently. Ultra-cooled Photomultiplier Tube and : Silicon photodiode. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. #PLUS 384) Methods. They perform rapid throughput cell-based assays while. USA. Add 10 µL of ADP Detection Mix to those rows. 150 µL of deionized water was pipetted into each well of a clean 96-well plate. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for. SpectraDrop High-Throughput Screening (HTS) Kit. Shaking: Linear and Orbital. After reading the ELISA plate on both readers, each standard curve was nearly identical. book Page 1 Wednesday, October 12, 2011 4:56 PMSpectraMax Plus 384 Microplate Reader (Molecular Devices cat. Outer wells aligned for proper viewing with FLIPR; Ideal for miniaturized 384 well fluorescent. and the A1 corner of the plate adapter (Figure 1-2). Instructions for adding new plates: CSI/CP2: Add plate. Microplates for applications that require high sample recovery rates. Supersedes 1. the Gemini XPS reader. Fluorescence Microplate Reader Comparison Chart. Figure 1. Plate readers, also known as. In addition, the iD5 reader measures TRF and FP and. B. 039. Scan every well . Wash heads are made of Polyethylene Terephthlate (PET) with stainless steel probes to prevent corrosion. Values are averages from 5 clean plates, each filled with 200 µL water/well. Versa Max. Packed 25 plates per sleeve, 2 sleeves per case. This is equivalent to having 511 built-in filters, 384-well: 0. However, for greater flexibility, some instruments can accommodate 6- to 1536-well plate formats. Artel MVS Bundle DM Std/800TSNB Reader Details. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. RUO. The SpectraMax Paradigm reader with HTRF Detection Cartridge offers high-throughput screening capability with excellent read times and Z’ factors. The Raman plate reader was designed to simultaneously observe the Raman spectrum of 192 analytes loaded in 192 wells on a standard 384-well plate. 1 OD at 230 nm. The SpectraMax ABS Plus reader utilizes Molecular . Visible absorbance -. 384-well microplate compatible Well read area range: H7 to K22 Figure 1-3 24-Well Micro-Volume Sample Slide Figure 1-4. Molecular Devices products are warranted to meet the stated specifications. Abs). Includes PC and software. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, andSupplier: Molecular Devices. Every SpectraMax® 340 PC 384 microplate reader has an optical system built around a monochromator. QC tools and IQOQ services. Before you do a bottom read, or use high profile (6-well, 12-well, 24-well, or 48-well) plates, you must remove the plate adapter from the plate drawer. Dual injectors for kinetic assays. Catalog number: BC1384W. Unparalleled performance on a personalized platform. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. #4TI-0214) UltraVision Plate 96 (Brooks Life Sciences cat. T = I/I 0 and %T = 100 (T). QuickSync. For more sample throughput, the system canabsorbance plate readers. 384 Well Plates; Wavelength(s): 200 to 1000 nm (Abs), 250 - 850 nm (Fluor) Specifications/Features: Shaking, Temperature control, Cuvette port; Read More. Products : Ionworks HT, Ionworks Quattro. The small-footprint Multiwash+ Microplate Washer quickly washes with both 96 and 384-well plates, has 20 standard wash protocols, and a simple LCD screen interface to customize speed, volume, soak time, and aspiration settings. Loading Buffer was also added to a set of cell-free wells for background controls. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. In addition, SoftMax Pro SoftwareThe Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. Simultaneous dual emission detection enables read times of only 2:17 for an entire 384-well plate. Data for a 384-well plate can be collected in less than two minutes, as opposed to up to 30The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Important things to consider when purchasing a multimode microplate reader. x. 2. Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96-. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. Overview of 384 Well SBS. 984. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Modefor the SpectraMax Multi-Mode Microp late Reader, as well as all other Molecular Devices' microplate readers. The plate reader consisted of a large-area illumination unit and a parallel detection unit; see Fig. In addition, SoftMax Pro SoftwareAfter incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. For more sample throughput, the system canThe SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. Dimensions: 18. 5 pM 384-well (50 µL) 6 pM 10 pM 1536-well (8 µL) 19 pM 25 pM 0200-7015 (LUM 384. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. Rating: 3. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. 6% for the on-board optics (monochromators). 20 plates/hour (2-minute read time) 96-well 1,920 data points/hour 384-well 7,680 data points/hour 1536-well 30,720 data points/hour Bar code manual mode Keyboard entry Bar code remote mode Short or long side Temperature control Ambient +5°C to 40°C General specifications Dimensions (in. For more sample throughput, the system canPage 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. The assay methods covered include. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. 96-384. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. 384 well plates 6–384 wells 6–384 wells 6–384 wells 6–1,536 wells (fluorometry, TRF, luminometry, AlphaScreen assays). 8 mm distance between well bottoms to plate bottomValidated by Molecular Devices for the FLIPR reader. x. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. 6, 12, 24, 48, 96, 384-well plates : ANSI/SLAS Light source. 6-384. Simultaneous dual emission detection enables read times of only 2:17 for an entire 384-well plate. 5 ng per well. Programmable 10% to 100%: Sample Cell Options: 96-, 384- or 1536-well microwell plates. SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. Temperature control (RT+3°C – 42°C) Liquid dispensers with reagent heater and stirrer. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. Results shown are from the SpectraMax M5 microplate reader, but similar results are obtained with other Molecular Devices readers with fluorescence detection mode (data not shown). Fluorescent Imaging Plate Reader (FLIPRTM ) technology to measure Ca2+ flux in cells was an important development in the early 1990’s and has played a significant role in HTS and lead optimization applications. (That difference comes at a cost, Amouretti explains, as higher plate densities require better optics and more sensitive detectors. Application Area: Spectrophotometer for molecular biology. This chapter reviews key criteria for microplate selection, including: well number, well volume and shape,. With the FLIPR 384 , you get the sensitivity, data fidelity, and real-time, kinetic information of the FLIPR I system in a 384-well mode. Use the provided 3. 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 µL per well. The systems can handle any slide-based microarray study, from small one- or two-fluor applications to multiple-fluor high-throughput projects requiring automated. For a higher . 384-well plates • Automated rinse reduces clogging • Quiet and efficient. EIGHT-CHANNEL OPTICS Plate reads as fast as five seconds MICROPLATES OR CUVETTES Use a 96- or 384-well microplate or a standard cuvette SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. 96- and 384-well. VMax . Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. TNF standard curve. Full-skirted standard or extra. You should ensure that there is enough volume in compound plate wells to operate the IonWorks® system taking into account the dead volume. Top. Select Yes at the warning. This can increase throughput and efficiency, thus saving time and space in the lab. VMax . #ABS Plus). 0 . Efficiency. 384-well format. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Align the two slots on the bottle holder with the two pegs on the rail. 8 mm distance between well bottoms to plate bottomValidated by Molecular Devices for the FLIPR reader. ,LTD (CAT#: STEM-S. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. 5 fg per well to 0. 96-1536. The user-friendly MultiWash™+ Microplate Washer from Molecular Devices, one of the pioneers in microplate detection, includes 20 preset protocols, works with both 96- and 384-well plates, and comes with 4 different wash/rinse bottles for out-of-the-box use. M2. 96/384-well plate formats. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. 96-deep-round-well-plate, 384-deep-square-well-plate, 24-deep-well-plates. The color touch screen provides a visual user interface, making programming fast and intuitive. 96- and 384-well . Molecular Devices Spectramax. 2. Features. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. 6-well tissue-culture treated (TC) plate, sterile. The BioTek 800 TS absorbance reader is an affordable, high-quality microplate reader for assays in 6- to 384-well formats. MultiWash+ Microplate Washer. 1536-well plates < 100 amol (384-well) 1 HTRF • Cisbio HTRF detection with optimized Xenon light source and . Learn how the PathCheck feature can be applied to protein quantitation experiments using Molecular Devices absorbance-based microplate readers. 1. Source plate capacity; 1 x plates. 012 fmol 0. In general microplate washers are designed to leave only 2 microliters of residue per well and operate with an accuracy of 3% CV per well and a uniformity of 4% CV across a plate. All stand-alone instrument functions can be controlled using the software. X. Typically, the full spectrum of signal from. 384-Well SBS Microplate, clear, square well, flat bottom, sterile, with cover, working volume 90 µl, packaged in sleeves of 10. Sensitivity and dynamic range are demonstrated by the high Z’ factors obtained on the SpectraMax. 과 같은 Parameter를 검사하여 평가할 수 있습니다. For more sample throughput, the system canThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Using Molecular Devices microplate readers and SoftMax. SpectraMax® ABS Plus microplate reader can run both cuvette-based and microplate reader applications on the same instrument. Two high-energy xenon flash lamps: Reading capabilities. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The ImageXpress® Pico Automated Cell Imaging System is more than a digital microscope, combining high-resolution imaging with powerful analysis. Time-resolved phosphorescence. 5. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The 384-well plate is limited to a maximum volume of a 30 µl addition in a much smaller diameter well, and using the 96. The fluid surface in a 384-well is a deeply curved meniscus. x ABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. Plate Formats: 6- to 384-well plates, NanoQuant Plate (Sold Separately) Detection Modes: Luminescence, Fluorescence, Absorbance. 340 PC384. Dynamic range > 6 decades . Typical 4 min/ 384 2 min/384 8 min/1536 Throughput plate plate plate Fully enabled protocols = reduced setup time. Description: The SpectraMax® L microplate reader measures flash and glow luminescence assays in both 96- and 384-well plates. Minimum volume and maximum throughput with 96- or. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. The Standard Thermo Scientific 384-well plate, Barcoded, White, is compatible with the majority of 384 block PCR and sequencing instruments. 0 out of 5 $ 8,495. 96-, 384-, and 1536. VMax . Video Description: The video below gives an overview of the Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader, and also demonstrates its functionality. x. *For Corning UV plates, mean values at 260 nm ranged from 0. Plate readers avoiding cross-talk are recommended for luminescence measurement in 384 well plates. Outer wells are aligned for proper viewing with FLIPR. These robust readers have been placed in labs from Antarctica to the. 5–25 µl. #0200-7011) Instrument setup The results above revealed excellent detection limits and dynamic range in all three Molecular Devices microplate luminometers. The absorbance was then read on the SpectraMax microplate reader at 426 nm, and the resulting OD values for all wells were averaged together to obtain a value of 0. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Dilution series for ZsGreen (green), AcGFP (blue) and DsRed (red) transfected cells. 3. Temperature control. x. For more sample throughput, the system canPlate Background OD, which was used for subsequent tests, was measured as follows. 50 μL total well volume, with recommended working volume of 5-40 μL. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. Once the assay runs its course, the final absorbance readout will take place on a SpectraMax® iD5 Multi-Mode Microplate Reader. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The VICTOR Nivo. the industry-leading microplate reader software for the SpectraMax Plus 384 system, allows complete customization of data collection and analysis. Some instruments can handle only 96-well plates and lower, whereas others can read up to the ultrahigh throughput densities of 1,536-well plates. FlexStation® 3 Multi-Mode MicroplateAlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. 6, 12, 24, 48, 96, 384-well plates : ANSI/SLAS Light source. Click SoftMax Pro Software User Guide. EPOCH2 Plate Reader/Spectrophotometer. Integrate any SpectraMax Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. Kinetic readings were started immediately with a Molecular Devices FlexStation Plate Reader. 8mm distance between well bottoms to plate bottom 50 µL total well volume, with recommended working volume of 5-40 µL High base plate design is compatible with automation and for bar coding Validated by Molecular Devices for the FLIPR reader. Turn the. Data for a 384-well plate can be collected in less than two minutes, as opposed to up to 30Molecular Devices Corporation (the “Seller”) shall not be liable for any incidental or consequential damages. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. 100 fmol luciferase per well. Tecan’s quality control and validation packages help our customers to fulfill all regulatory requirements for their Tecan microplate readers in an efficient, cost-effective way. MultiWash™+ Microplate Washer benefits include, walk-up usability, 4 wash and rinse. That explains the lower values on the LmaxII384 plate reader. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with. The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl. Get Quote . Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. The analyzed results are. : 2 Oct 12 Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. plates < 100 amol (384-well) 1 0200-7018. For FlexStation I/II/3 plate readers, what are the dead-volumes for the compound plate? The FlexStation plate readers assume a 1 mm pipette height from the bottom of the well when aspirating from a compound source plate. (Component A, C) is sufficient for 1 plate (96-, 384-, 1536-well). Sensitivity and dynamic range are demonstrated by the high Z’ factors obtained on the SpectraMax. Browse for different fluorescent readers below and compare across different instrument suppliers. Both the P and M injectors were set to deliver 100 µL (96-well plate) or 25 µL (384-well plate), with a 2-second delay and 10-second integration time. 100 fmol 1536-well (8 µL) 0. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. It also integrates with the. Four wash/rinse bottles are included and configurable for both 96- and 384. If an organoid line forms small organoids in the 24-well semi. On the FlexStation 3 reader, the entire series of reactions is monitored in real time with simultaneous on-board reagent pipetting and luminescence detection (Figure 1). Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Find our technical support information along with contact details. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader Multimode Readers Comparison Chart. 1 fmol luciferase per well. is a welcome addition to any lab that is routinely measuring concentration or absorbance in 96 and/or 384 well plates. The price of a microplate reader depends on the technical equipment and the number of measurement technologies that the device can measure. Reliable performance with a proven track record. For the 96-well plate, this equates to 1 fg per well to 1 ng per well; for the 384-well plate it is 0. It has a. SpectraMax iD5. +1 800. We would like to show you a description here but the site won’t allow us. 8 mm distance between well bottoms to plate bottom; 50μL total well volume, with recommended working volume of 5-40μL; High base plate design is compatible with automation and for bar coding; Validated by Molecular Devices for the FLIPR reader. Was the failure only of the Detection Estimate (Mag3) Test for the M3/M4/M5/M5e/Flex3?Gurard-Levin describes his lab’s microplate reader selection: “In our labs, specific and refined optics are important, as well as the ability to use high density plates including those with 384- or 1536-wells.